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KMID : 0359320150550010009
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Korean Journal of Veterinary Research
2015 Volume.55 No. 1 p.9 ~ p.12
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Comparison of transport media for the isolation and detection of Brachyspira hyodysenteriae
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Cho Se-Ji
Kim Jong-Wan
Kim Ha-Young
Oh Sang-Ik
Jeong So-Jeong
Jung Ji-A
Lee Myoung-Heon
Cho Ho-Seong
Byun Jae-Won
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Abstract
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Brachyspira (B.) hyodysenteriae is a causative agent of swine dysentery that is responsible for death and economic losses in the pig industry. It is imperative that clinical samples be delivered fresh for accurate diagnosis. The viability and DNA detection of B. hyodysenteriae using lab-made (phosphate buffered saline and modified tryptic soy broth) or commercial transport media (C, D, and E) were compared by culturing and real-time PCR at 4oC or room temperature (RT), respectively. B. hyodysenteriae grown in D (Anaerobe Systems, USA) and E (Starplex Scientific, Canada) media was viable for 4 days at 4oC and RT. However, B. hyodysenteriae in A, B, and C (culture swab; BD Biosciences, USA) media were not recovered after 2 days at RT. Ct values for real-time PCR at 4oC and RT ranged from 27.2 ± 2.1 (C) to 29.6 ± 0.5 (B), and 28.0 ± 0.9 (E) to 30.2 ± 1.5 (B), respectively. Considering the field conditions, it is important that transport media is used for specimen isolation and PCR to obtain an accurate diagnosis of swine dysentery.
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KEYWORD
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Brachyspira hyodysenteriae, pigs, real-time PCR, transport media
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